Diabetes mellitus is a chronic metabolic disorder that adversely affects male reproductive organs, resulting in infertility due to testicular atrophy. Hyperglycemia and oxidative stress are considered the main factors implicated in the pathogenesis of diabetic complication. Hesperidin is a natural flavonoid with wide pharmacological effects such as hypoglycemic, antioxidant and anti-inflammatory. This work aims to study the ability of hesperidin to counteract diabetes-induced testicular alterations. 50 rats were used in this experiment, divided equally and randomly into five groups: control C, diabetic DM, diabetic +hesperidin DM+H, diabetic +insulin DM+In and hesperidin group H. At the end of the experiment, the rats were sacrificed and evaluated for body weight, blood sugar level, testicular weight, histopathological, immunohistochemistry, morphometric analyses, and spermatozoa analysis. Results revealed that DM significantly reduced the body and testicular weights, produced drastic histopathological, morphometric adverse changes of the testicular tissues and morphological abnormalities of the spermatozoa. Hesperidin produced valuable hypoglycemic effect, increased the body and testicular weights, ameliorated the histopathological changes, restored normal germinal epithelium and spermatogenesis, restored morphometric parameters and significantly decreased the morphological abnormalities of the spermatozoa caused by DM. Insulin could improve some parameters that were adversely changed by DM, but less than the hesperidin. We conclude that treatment with hesperidin appeared to be more effective in counteracting the toxic effects of diabetes on testes than insulin.
Maha S. Abd Elsamie, Mona H. Mohammed Ali, Omayma M. Mahmoud, Eman M. Kamel
Anatomy Department, Faculty of Medicine, Suez Canal University, Egypt
Eur. J. Anat.
ISSN 2340-311X (Online)