The acute exocrine pancreatic injury was induced by L-arginine in rats and the potential prophylactic/therapeutic role of probiotics was evaluated in a histological, immunohistochemical, and molecular study. Thirty adult male rats were used and randomly divided into five groups. Group I (Control group). Group II (Probiotic group): supplemented orally with 2X108 CFU/kg Lactobacillus Acidophilus for 5 days. Group III (Acute pancreatitis, AP) group: received double intraperitoneal injections of 250mg/100g BW L-arginine with one hour apart. Group IV (Prophylactic group): before induction of AP, the rats were supplemented orally with 2X108 CFU/kg Lactobacillus Acidophilus for 5 days. Group V (Therapeutic group): after induction of AP, the rats were supplemented orally with 2X108 CFU/kg Lactobacillus Acidophilus for 5 days. Pancreatic specimens were processed for histological and biochemical studies, and assessment of deoxyribonucleic acid (DNA) fragmentation by gel electrophoresis. Serum amylase, lipase and IL-1b were determined. Also, pancreatic malondialdehyde (MDA), reduced glutathione (GSH) and myeloperoxidase (MPO) were assessed. In AP group, the histological architecture of the exocrine pancreas was distorted with acinar cell necrosis, inflammatory cellular infiltration, interstitial edema, and hemorrhage. Inducible nitric oxide synthase (iNOS) was highly expressed in the cytoplasm of acinar cells. Moreover, AP group displayed much DNA fragmentations and a significant increase of amylase, lipase, IL-b, MDA, MPO, and a decrease of GSH. Most deleterious effects of the AP were alleviated more with the prophylactic than therapeutic use of Lactobacillus Acidophilus. In conclusion: The prophylactic regimen of probiotics has a supreme beneficial effect over the therapeutic one in ameliorating the AP.
Dalia A. Mandour, Asmaa M. Tolba
Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Postal Code: 44519, Egypt
Eur. J. Anat.
ISSN 2340-311X (Online)