The objective of this study was to analyze the potential hepatotoxicity of the doxorubicin (DOX). 50 male albino rats were treated with doxorubicin daily for 30 days. Hepatotoxicity was monitored by quantitative analysis of the serum alanine aminotransferase (ALT), Gamma-glutamyl transferase (γ-GT) activities, total protein, and albumin. The second aim of this study to investigate affected chlorpyrifose or glyphosate alone or together on lipid profile levels of cholesterol, triglyceride, low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) respectively, Triiodothyronine (T3), thyroxin (T4) and thyroid-stimulating hormone (TSH) were measured, and livers were collected for histopathological study for light and electron microscope. The results testify to significant elevation liver functions (ALT and GGT), as well as significant decrease in total protein and albumin level. The hormones registered a significant decrease in T3 and T4 while there is an increase in TSH. Histopathology revealed vacuolar of hepatocytes with random hepatocyte necrosis and mononuclear cell infiltration. The administration of the MSCs and placenta extract have beneficial and decrease side-effects against the deleterious changes of Doxorubicin. Histopathology revealed degeneration vacuolar of hepatocytes with random hepatocyte necrosis and mononuclear cell infiltration. The administration of the MSCs and HPL had beneficial and decrease side effects against the deleterious changes of DOX. In conclusion, results suggest a potential contribution of DOX to the etiology of some diseases, while MSCs and PE have beneficial effects, as they tends to dampen DOX toxicity in rats.
Alsayed A. Abdelhady1, Mahmoud. Diab2, Ahmed Nabeeh3
1 Anatomy and Embryology Department, Faculty of Medicine, Helwan University Cairo, Egypt
2 Anatomy and Embryology Department, Faculty of Medicine. Al-Azhar University Cairo, Egypt
3 Zoology Department, Faculty of Science, Al-Azhar University Cairo, Egypt