European Journal of Anatomy

Official Journal of The Spanish Society of Anatomy
Cover Volume 15 - Number 1
Eur J Anat, 15 (1): 47-72 (2011)

A rat model of oligomeric forms of beta-amyloid (A√?) peptide: neuronal loss, synaptic alteration, astrogliosis, and calcium-binding proteins activation in vivo

Alicia Gonzalo-Ruiz1, Maria Delso1, Isabel Carrero1, Pilar Gonzalo Vicente1, José Miguel Sanz-Anquela2, Manuel Rodríguez3, Juan Arévalo-Serrano2

1Laboratory of Neuroanatomy, Institute of Neuroscience of Castilla and Le√≥n, University of Valladolid (Campus ‚??Duques de Soria‚?Ě), 42004-Soria, Spain, 2Department of Medicine, Hospital Pr√≠ncipe de Asturias, Alcal√° de Henares, Madrid, Spain, 3Department of Biochemistry, University of Barcelona, Spain.

ABSTRACT Oligomers of Beta-amyloid (A√?) peptide are presumed to cause synaptic and cognitive dysfunction in Alzheimer‚??s disease (AD). However, their contribution to other pathological features of AD remains unclear. To address the latter, we applied microinjections of A√?1-42 oligomers into the retrosplenial cortex of the rat. We observed that A√?1-42 induced a greater reduction in neuronal density as compared with that seen at control (A√?42-1) injections. Oligomers of A√?1-42 peptide caused synaptic alterations, as evidenced by the decrease in the presynaptic marker synaptophysin and the increase in chromogranin A. We also detected a marked interaction between GFAP-, and A√?immunoreactive material in a time-dependent manner. To address the possible mechanisms involved in astrocyte activation, we analyzed the interaction between the calcium-dependent protease, calpain-1, and the calcium-binding protein, S100B, and astrogliosis in response to A√? toxicity. Calpain-1 activation was studied by immunoblotting. Three immunopositive protein bands (80kDa, 76kDa, and 18kDa) were detected. Densitometry analyses revealed a significant increase in calpain-1 at 76kDa and at position 18kDa in A√?1-42-treated animals as compared with the corresponding bands in control animals. Confocal analysis showed codistribution of A√?-, and calpain 1-immunoreactivities in cortical cells, and in reactive astrocytes surrounding the injection of A√?, and both cortical and leptomeningeal blood vessels. A colocalization of GFAP and S100B proteins was observed in astrocytes that surrounded the A√? injection, and also in reactive astrocytes in close association with blood vessels. In conclusion, our results suggest that calpain-1 and S100B might play a critical role in astroglisois in response to A√? toxicity.

Keywords: Alzheimer‚??s disease, Retrosplenial cortex, Astrocytosis, S100B, Calpain-1

European Journal of anatomy
ISSN 2340-311X (Online)