In this study, the effects of diabetes mellitus on stereological changes of sperm parameters in mice were investigated. Mice under standard housing conditions were assigned into two experimental groups: (I) control (II) diabetic (N = 6 mice per group). The sperm samples were collected from the right cauda epididymis of the mice for measuring the sperm and sperm count. The volume of the sperms’ heads was estimated using the nucleator method. The length of the sperms flagellum and mid-piece was estimated by counting the number of intersections of the tails and Merz grid test line in an unbiased counting frame, superimposed on live images of sperms. The results showed that the total sperm count and sperm motility in Streptozotocin (STZ)-induced animals was decreased, in comparison with the control groups. Our results showed a significant difference in the volume and surface area of the head and the length of the flagellum between the sperms in the control and diabetic groups. In conclusion, our results indicated that type 1 diabetes induced impairment in sperm parameters.
Diabetes mellitus induced impairment of sperm parameters in mice: A stereological method
Sara Dadras1,2, Mohammad Bayat2, Marefat G. Novin1,2, Hamid Nazarian1, Amir Raoofi3,4, Shabnam Abdi5, Sina Sadeghzadeh6, Faezeh Tajari7, Mohammad-Amin Abdollahifar1,2
1 Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Anatomical Sciences, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
4 Leishmaniasis Research Center, Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran
5 Department of Anatomical Sciences & Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
6 Bachelor’s degree, Cognitive Neuroscience and Evolutionary Psychology, Harvard University, Cambridge, Massachusetts, USA
7 Student Research Committee, School of Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
