TY  - JOUR
A1  - ,  
T1  - Cryopreservation can induce transitory changes in the morphology and differentiation markers of rhabdomyosarcoma cells
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 1998
VL  - 2
SP  - 109
EP  - 114
UR  - http://www.eurjanat.com/web/paper.php?id=98020011
KW  - Myogenic differenriarion - crearine kinase isoenzyme - intermediate filaments - rhabdomyosarcoma cells
N2  - Weinvestigated whether cryopreservation rnodi­ fíes the biochemical and morphological parame­ ters of diffcrentiarion (creatine kinase isocnzy­ mes and desmin and vimentin molecules) in rhabdomyosarcorna cells, used as an experirnen­ tal model to analyse myogenic differenriation rnechanísrns. Following cryopreservation of two rhabdomyosarcorna cell lines (A-673 and A-204) using DMSO, the analyses revealed changes in thc expression of differentiation markers over the firsr three days of culture lncreases in the expression of crearme kinase-MM and crearme kinase-Mls fractions and in desmin were accorn­ panied by a decrease   in  virnentin    and  creatine kinase-Bls   cxpressíon    in  both   rhabdornyosarco­ ma cell  lines.   l lowever,    no  modifications   were Iound   in  the   sarne   period    when    glycerol    was used lO cryopreserve    rhe cells.  On  rhe fourth   day"  of culture   after  cryopreservation       with   D~ISO  anormal  pauern   of  expression    of rhese  molccules was  found.    Our   analyses    demonstrate     that   the cryopreservation       procedure      may    modify     the degree   of  differentiation       in  rhabdornyosarcoma cells  and  suggcsr  (har (his  effecr  may  be  induced by  DM   O.  These   results  are  of  interest   to  those\Vorking   \Virh  myogenic    cells.  in particular    rhose srudying   diffcrenriarion     mechanisms.
ER  -