TY - JOUR A1 - Perez-Garcia, C. G. A1 - Carmona-Calero, E. M. A1 - Gonzalez-Marrero, I. A1 - Castaneyra-Ruiz, L. A1 - Gonzalez-Toledo, J. M. A1 - Castaneyra-Ruiz, A. A1 - De Paz-Carmona, H. A1 - Castaneyra-Martin, M. A1 - Meyer, G. A1 - Castaneyra-Perdomo, A. T1 - Immunohistochemical study of Pax6 and Reissner fibre expression in the prenatal development of the mouse subcommissural organ JO - Eur. J. Anat. SN - 1136-4890 Y1 - 2009 VL - 13 SP - 111 EP - 115 UR - http://www.eurjanat.com/web/paper.php?id=09030111 KW - glycoprotein KW - transcription factor PAX6 KW - article KW - brain development KW - brain ventricle KW - cell differentiation KW - cell migration KW - cell proliferation KW - cerebrospinal fluid KW - embryo KW - ependyma cell KW - heterozygote KW - homozygote KW - immunohistochemistry KW - mouse KW - nonhuman KW - prenatal development KW - protein expression KW - protein secretion KW - subcommissural organ KW - thalamus N2 - The subcommissural organ (SCO) releases glycoproteins into the ventricular cerebrospinal fluid (CSF), where they form Reissner's fibre (RF) and also secretes a CSF-soluble material different from RF-material. Pax6 is a transcription factor important for the regulation of cell proliferation, migration and differentiation in the developing brain. In the present work, we studied wild-type, heterozygous and homozygous Sey mice to compare the expression of RF-antibody and Pax6 in the SCO and adjacent structures. In wild-type mice between E15 to E18, we observed Pax6 expression in cells surrounding the secretory cells of the SCO, and RF-immunoreactive material only in the SCO ependymal cell layer and its basal process. In the heterozygous mice, the neuroanatomical structure of the SCO was present, but RF-antibody staining and Pax6 expression was scarce or almost undetectable; in the homozygous mice neither SCO nor other epithalamic structures were found. We suggest that Pax6 expression at the periphery of the SCO is essential for the development and activity of the organ. ER -