TY  - JOUR
A1  - ,  
T1  - Application of stained blood cells for the differentiation of arteries and nerves in cadavers
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2006
VL  - 10
SP  - 97
EP  - 100
UR  - http://www.eurjanat.com/web/paper.php?id=06030097
KW  - formaldehyde
KW  - glutaraldehyde
KW  - anatomical variation
KW  - artery
KW  - article
KW  - blood cell
KW  - controlled study
KW  - forensic identification
KW  - functional assessment
KW  - human
KW  - internal iliac artery
KW  - microscopy
KW  - morphology
KW  - nerve cell
KW  - staining
N2  - The demonstration of the functional arteries to first year medical students in the dissection room is very important, but after cadaver fixation with formalin and other preserving materials the arteries will be empty and the fixative material will take the place of blood. Thus, the arteries will be colorless and students will find it hard to differentiate between arteries and nerves. The aim of this study was to stain arteries and differentiate them from nerves in cadavers by means of an easy method, the use of stained blood cells. Whole blood cells (packed cells) were initially treated with a 1% glutaraldehyde solution, washed with PBS and then stained with safranin. 10 ml of stained blood cells was injected into the internal iliac artery of the cadaver. The stained blood cells injected into the internal iliac artery differentiated the internal pudendal arteries from the pudendal nerve and the inferior rectal artery from the inferior rectal nerve. The preparation of stained blood cells and their application could be used by all medical and dental students to facilitate learning of the functional arteries.
ER  -