TY  - JOUR
A1  - ,  
T1  - Auto-paracrine regulation of the proliferation of pituitary ACTH-producing cells demonstrated by immunoblockade of IL-1?
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2006
VL  - 10
SP  - 7
EP  - 13
UR  - http://www.eurjanat.com/web/paper.php?id=06010007
KW  - corticotropin
KW  - cycline
KW  - interleukin 1beta
KW  - animal cell
KW  - animal experiment
KW  - animal tissue
KW  - apoptosis
KW  - article
KW  - cell culture
KW  - cell proliferation
KW  - cell size
KW  - controlled study
KW  - corticotropin release
KW  - hormone release
KW  - hypophysis
KW  - hypophysis cell
KW  - immunohistochemistry
KW  - in vitro study
KW  - male
KW  - nonhuman
KW  - paracrine signaling
KW  - rat
N2  - IL-1? is an interleukin synthesised in the pituitary gland and is involved in the stimulation of ACTH secretion. However, to date it has not been determined whether IL-1? is able to regulate the proliferation of pituitary ACTH-producing cells, like other regulatory factors involved in the regulation of ACTH such as vasopressin or CRH. The aim of the present study was to address whether IL-1? is involved in regulating the proliferation and apoptosis of pituitary ACTH-secreting cells. Thus, we performed an in vitro study on monolayer cultures of rat pituitary cells, neutralising the possible paracrine effect of IL-1? by immunoblockade of the interleukin and later determining the degree of proliferation or apoptosis of ACTH-secreting cells. The effects of immunoblockade were validated by determining the modifications in the patterns of the immunohistochemical reaction to ACTH-positive cells. Immunoblockade of IL-1? decreased the percentage and proliferation of ACTH-positive cells at all time-points analysed, mainly between 6 and 12 h (p < 0.01). Moreover, immunoblockade decreased the sizes of the cellular and nuclear areas at all time-points studied, with significant decreases (p < 0.01) after 3, 6 and 12 hours. The results obtained suggest that -in the same way as it regulates the secretion of the hormone- IL-1? could be involved in regulating the proliferation of ACTH cells.
ER  -