TY  - JOUR
A1  - ,  
T1  - Alteration of cholinergic, excitatory amino acid and neuropeptide markers in the septum-diagonal band complex following injections of fibrillar ?-amyloid protein into the retrosplenial granular cortex of the rat
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2002
VL  - 6
SP  - 95
EP  - 107
UR  - http://www.eurjanat.com/web/paper.php?id=02020095
KW  - amyloid beta protein
KW  - biochemical marker
KW  - excitatory amino acid
KW  - glutamic acid
KW  - neuropeptide
KW  - neurotensin
KW  - substance P
KW  - Alzheimer disease
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - cholinergic nerve
KW  - controlled study
KW  - diagonal band of Broca
KW  - female
KW  - forebrain
KW  - immunocompetent cell
KW  - intracerebral drug administration
KW  - neurochemistry
KW  - neurotoxicity
KW  - nonhuman
KW  - rat
KW  - septum nucleus
N2  - The effects on cholinergic, excitatory amino acid and neuropeptide markers in the basal forebrain of an intracerebrally-injected synthetic peptide corresponding to the first 1-40 amino acids of beta-amyloid protein (A?1-40) was studied in rats. Focal deposition of A? in the retrosplenial granular cortex resulted in a significant loss of cholinergic and glutamate-immunoreactive neurons in the septum-diagonal band complex. The medial septal nucleus showed a reduction of 39% ± 1% in the number of cholinergic neurons and a significantly reduced number of cholinergic neurons (up to 22% ± 10%) was also found in the horizontal nucleus of the diagonal band of Broca in comparison with the same paramaters in uninjected controls. A markerd loss of glutamate-containing neurons was also found in the medial septal nucleus (up to 19% ± 6%) together with a decrease of approximately 22% ± 3% in the horizontal nucleus of the diagonal band of Broca when compared with uninjected control animals. Furthermore, a moderate reduction in fibres and structures such as terminals immunoreactive for glutamate, substance P, and neurotensin was observed in the septum-diagonal band complex. These results show that two different neurochemically defined populations of neurons in the basal forebrain are affected by the neurotoxicity of A? in vivo.
ER  -