TY  - JOUR
A1  - ,  
T1  - Localization of excitatory amino acid and neuropeptide markers in neurons of the subicular complex projecting to the retrosplenial granular cortex of the rat
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2001
VL  - 5
SP  - 119
EP  - 132
UR  - http://www.eurjanat.com/web/paper.php?id=01030119
KW  - 4 aminobutyric acid
KW  - cholera toxin B subunit
KW  - excitatory amino acid
KW  - glutamic acid
KW  - horseradish peroxidase
KW  - leucine enkephalin
KW  - neuropeptide
KW  - neurotensin
KW  - neurotransmitter
KW  - substance P
KW  - animal tissue
KW  - article
KW  - cingulate gyrus
KW  - controlled study
KW  - female
KW  - hippocampus
KW  - histochemistry
KW  - immunohistochemistry
KW  - limbic system
KW  - male
KW  - nerve cell
KW  - nonhuman
KW  - rat
KW  - subiculum
N2  - Retrograde labelling was combined with immunohistochemistry to localize neurons containing glutamate and different neuropeptides such as neurotensin, leu-enkephalin, and substance P-like immunoreactivity in the projection pathways from the presubiculum and subiculum to the retrosplenial granular cortex of the rat. Injections of horseradish peroxidase conjugated to subunit B of cholera toxin (CT-HRP) into the retrosplenial granular cortex labelled large numbers of neurons in the presubiculum. A significant number of retrogradely-labelled neurons was seen in the dorsal subiculum, whereas small numbers of CT-HRP-labelled neurons were also found in the ventral subiculum. In the presubiculum, 90-95% of the CT-HRP-labelled neurons (30-32 per section) were also immunoreactive for glutamate, and small numbers of retrogradely-labelled neurons also displayed neurotensin-, leu-enkephalin- or substance P-immunoreactivity. In the subiculum, approximately 90-95% of the CT-HRP-labelled neurons (19-20 per section) were also immunoreactive for glutamate, and a significant number of retrogradely-labelled neurons (70-75%, 14-15 per section) also displayed neurotensin immunoreactivity. In addition, small numbers of CT-HRP-labelled neurons in the subiculum were immunoreactive for leu-enkephalin or substance P. These results suggest that the complexity of the neurotransmitter(s)/neuromodulator(s) of the subicular projections to the retrosplenial granular cortex of the rat should be taken into account when considering the mechanisms of the retrosplenial cortical neurons thought to play a role in memory.
ER  -