TY  - JOUR
A1  - ,  
T1  - Innervation of the chicken esophagus wall: Localization and distribution of the acetylcholinesterase-positive nervous elements and ultrastructure
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2000
VL  - 4
SP  - 103
EP  - 109
UR  - http://www.eurjanat.com/web/paper.php?id=00020103
KW  - acetylcholinesterase
KW  - tolonium chloride
KW  - animal cell
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - chicken
KW  - esophagus
KW  - ganglion
KW  - immunohistochemistry
KW  - innervation
KW  - nerve plexus
KW  - nonhuman
KW  - transmission electron microscopy
KW  - ultrastructure
N2  - We have studied the distribution pattern of the acetylcholinesterase-positive innervation of the four-week-old chicken esophagus. We localized the different innervation plexuses and their inter-connections on sections of tissue samples. Later, we observed the three-dimensional structure of the plexuses by means of histochemical methods on whole-mount preparations, after delaminating the esophagus wall. We also studied the components of the nervous plexuses by optic microscopy on semi-thin cuts stained with toluidin blue, and studied their ultrastructure by transmission electron microscopy. In the myenteric plexus we observed compact ganglions between both muscle layers, as well as intra and inter-fascicular neuronal cell bodies in the circular muscle layer. Nervous trunks and fibers distribute along the connective tissue among the muscular bundles. The submucous plexus was found to be less abundant in nervous elements titan the myenteric plexus, and its ganglions were smaller. In the lamina propria abundant AChE+ fibers, which innervate the glands at their base, and in the surroundings of the tubules up to the epithelium were seen. On whole-mount preparations we describe that the ganglions of the myenteric plexus are larger and form a denser network than in the submucous plexus. TEM revealed varicosities with cholinergic, adrenergic, peptidergic and mixed vesicles.
ER  -