European Journal of Anatomy

Official Journal of The Spanish Society of Anatomy
Cover Volume 23 - Number 6
Eur J Anat, 23 (6): 405-413 (2019)

Parsley against nicotine toxicity

Shymaa Mohamed Amer El-fiky1, Hanaa Zakaria Nooh1, Gehan Farouk AliEl-Akabawy1,2, Noha Mohey Issa1

1Department of Anatomy and Embryology, Faculty of Medicine, Menoufia University, Menoufia, Egypt2Department of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, KSA

ABSTRACT Nicotine exposure during pregnancy is linked to multiple obstetrical, fetal, and developmental com-plications. Parsley (Petroselinum crispum) is an aromatic herb, which has well-known potent anti-inflammatory and anti-oxidative effects. The pur-pose of this study was to investigate, for the first time, the protective effect of parsley extract on al-veolar stage of lung development in rats exposed to perinatal nicotine. Thirty Sprague-Dawley adult female rats were randomly divided into five main groups after being pregnant: control, sham control, parsley-treated (5mg /kg/day), nicotine-treated group (1mg /kg/day), and protected (nicotine + parsley extract) groups. Nicotine was injected sub-cutaneously, while parsley extract was given orally by gastric tube from the 7th day of pregnancy until the 21st day postnatally. At the end of the experi-ment, lungs of 21-day-old male offspring were sub-jected to biochemical, histological, and immuno-histochemical analyses. Our results revealed toxic effects of nicotine on alveolar stage of rat lung de-velopment. These were indicated by histopatholog-ical alterations, including poorly developed primary and secondary septa; interstitial tissue infiltration with inflammatory cells, atypical features appeared in some cells of bronchioles and blood vessels. In addition, a reduction in elastic fibers contents and in alpha smooth muscle expression, an increase in surfactant protein B expression, and changes of oxidative stress indices and tumor necrosis factor alpha level in lung tissue were detected. Co-administration of parsley extract ameliorated nicoti-ne-induced toxic alterations on the development of the lung. Therefore, parsley can be a promising candidate for the prevention of nicotine-induced toxicity in the developing lung.

Keywords: Antioxidant Secondary septation Alveolar myofibroblast Lung Nicotine

European Journal of anatomy
ISSN 2340-311X (Online)