TY  - JOUR
A1  - ,  
T1  - The Goldi-method for electron microscopy. A X-ray microprobe study
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 1997
VL  - 1
SP  - 53
EP  - 65
UR  - http://www.eurjanat.com/web/paper.php?id=97020034
KW  - Golgi-method
KW  - electron microscopy
KW  - neurocytology
KW  - X-Ray microanalysis.
N2  - Since its introduction by Stell in 1964, the "Golgi method for electron microscopy" has achieved considerable technical improvements, particularly with the so-called de-impregnation procedures such    as the gold-toning. In the present study the chemical composition of the precipitantes produced by de-ímpregnation of previously Golgi-rapid stained neurons and glial cells was investigated by X-ray microanal­ ysis. Following de-impregnation procedures were considered: gold-toníng, gold-toning after exposition of the tissue to white light and treat­ ment with a photographic developer with or without gold-toning. Golgi stained ce lis without any treatment served as control. The micro­ probe studies reveal that the precipitates con­ tain both silver and gold after gold-toníng. A complete substitution of the silver located in the silver chromate crystals was only achieved in sections exposed to white light before gold­ toning. After treatment with a photographic developer (chemical reduction), the silver chro­ mate crystals disappeared and silver precípitates of different sizes were found instead. After gold-toníng, the substitution of silver in these precipitates is only partial. As a result of the fix­ ation of the tissue, osmium, uranium and chro­ mium were found in all situations investigated. These findings were discussed in relation with possible chemical mechanisms     involved in the de-impregnatíon procedures.
ER  -