TY  - JOUR
A1  - ,  
T1  - Cytoprotective activities of Anacardium occidentale (Linn) on the testis of streptozotocin-induced diabetic rats
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2013
VL  - 17
SP  - 92
EP  - 101
UR  - http://www.eurjanat.com/web/paper.php?id=120111vu
KW  - <i>Anacardium occidentale</i>
KW  - Testes
KW  - Cytoprotective
KW  - Streptozotocin
KW  - Reproductive hormones
KW  - Hyperglycaemia 
N2  - This study presents the protective effects of methanolic <i>Anacardium occidentale</i> leaf extract (AOLE) on the testes of streptozotocin (STZ)-induced diabetic rats. Forty male wistar rats (<i>Rattus norvegicus</i>) were randomly divided into four experimental groups A,B,C and D. Hyperglycaemia was induced by a single intraperitoneal injection of 0.1 M STZ (70 mg/kg b.w). Five days after the confirmation of hyperglycaemia by using a glucometer (Roche<sup>(R)</sup>) and compatible glucose test strips, groups A and B were treated with 300 mg/kg b.w of the extract and 1 I.U/kg b.w insulin respectively. Groups C and D served as hyperglycaemic and normal controls and received 1 ml/kg b.w citrate buffer respectively.After 16 days of treatment, blood was collected through retro-orbital puncture for insulin and reproductive hormone (FSH, LH and testosterone) analysis; the animals were then sacrificed and the testes were processed for histological staining. Data obtained were expressed as means of ten (10) replicates ± SEM and subjected to one-way analysis of variance (ANOVA) and the Scheffeâ??s <i>post hoc</i> test for multiple comparisons.Results showed that the levels of testosterone, FSH and LH in the AOLE- and insulin-treated rats increased significantly compared to the hyperglycaemic controls (<i>P</i><0.05).Histological sections revealed improved cellularity, germinal epithelium, tubular diameter, cross-sectional area and luminal spermatids in the AOLE- and insulin-treated rats when compared with the hyperglycaemic control.AOLE improved the structural integrity of the testes, promoted spermatogenesis, and improved the profile of reproductive hormones.
ER  -