TY  - JOUR
A1  - M, Riesco-Lopez J.
A1  - R, Vazquez 
A1  - J, Carretero 
A1  - A, Juanes J.
A1  - M, Rubio 
A1  - E, Blanco 
A1  - M, Asensio 
A1  - M, Riesco J.
T1  - Variations of nNOS and its mRNA in the neurons of the hypothalamic supraoptic nucleus in response to stress by immobilization
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2009
VL  - 13
SP  - 11
EP  - 18
UR  - http://www.eurjanat.com/web/paper.php?id=09010011
KW  - messenger RNA
KW  - nitric oxide
KW  - nitric oxide synthase
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - controlled study
KW  - densitometry
KW  - hypothalamus
KW  - hypothalamus hypophysis system
KW  - immobilization stress
KW  - immunohistochemistry
KW  - in situ hybridization
KW  - male
KW  - morphometrics
KW  - nonhuman
KW  - nucleotide sequence
KW  - protein expression
KW  - rat
KW  - stress
KW  - supraoptic nucleus
N2  - Using rats subjected to immobilization stress, the aim of the present study was to investigate the expression of neuronal nitric oxide synthase and of its mRNA in the magnocellular neurons of the supraoptic nucleus of the hypothalamus, which does not have parvocellular neurons or direct neurohaemal connections with the anterior lobe of the hypophysis. nNOS expression was studied with immunohistochemistry using sheep anti-nNOS serum, and the intraneural detection of nNOS mRNA was accomplished using a non-isotopic in situ hybridization technique, employing a specific biotinylated probe. The acute stress elicited by restraint induced an increase in the overall size of the supraoptic nucleus, together with an increase in the number of magnocellular neurons expressing nNOS immunoreaction. The optical densitometry values of the nNOS immunoreaction, the nuclear areas of the immunoreactive neurons, and the density of neurons showing nNOS mRNA hybrids were higher in the supraoptic nucleus of the stress-treated animals than in the controls, suggesting that nitric oxide of nNOS origin plays an active role in the magnocellular neurons of the hypophyseal-hypothalamic system and the involvement of this system in the stress axis.
ER  -