TY - JOUR A1 - , T1 - Quantitative analysis of the human corpus callosum under light microscopy JO - Eur. J. Anat. SN - 1136-4890 Y1 - 2007 VL - 11 SP - 95 EP - 100 UR - http://www.eurjanat.com/web/paper.php?id=07020095 KW - tolonium chloride KW - adult KW - aged KW - article KW - controlled study KW - corpus callosum KW - female KW - glia cell KW - human KW - male KW - microscopy KW - nerve fiber KW - normal human KW - quantitative analysis KW - sex difference N2 - In recent years many workers have studied the morphology of the adult corpus callosum and controversy exists regarding gender- and age-associated differences. Callosal size may vary due to differences in the number and size of nerve fibers, glial cells and blood vessels. However, very little is known about the fiber composition of the human corpus callosum and how this might affect the length and thickness. The aim of this study is to investigate the regional differences in the numbers and diameters of several components of the corpus callosum per unit area and to determine age- and sex-related differences in the fiber composition of the corpus callosum. Eight human brains, both male and female, ages ranging from 25 years to 67 years, were studied. Semithin sections taken from different regions of the corpus callosum were stained with toluidine blue, examined under a Leitz DMRHC research microscope at a magnification of 40 x, and viewed at larger magnification on a computer screen after zooming. The number, diameter and area occupied by myelinated nerve fibers, glial cells and blood vessels per 0.01 mm2 were noted and analysed statistically. Thinly myelinated (diameter < 1.5 ?m) nerve fibers were abundant in the genu and the lowest number was noted in the body per unit area. The area occupied by glial cells was more greater in the body of the corpus callosum. The splenium had a greater number of thick fibers (> 5 ?m). The number of myelinated nerve fibers in the body of the corpus callosum was lower than in other regions. This may have been due to the presence of greater number of glial cells and large myelinated nerve fibers occupying a unit area. There was no statistically significant age- or sex-difference in the number of nerve fibers. ER -