TY  - JOUR
A1  - ,  
T1  - Effects of Arg-vasopressin and corticosterone on IL-1?-induced modifications of pituitary ACTH-producing cells
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2005
VL  - 9
SP  - 127
EP  - 134
UR  - http://www.eurjanat.com/web/paper.php?id=05030127
KW  - arginine
KW  - corticosterone
KW  - corticotropin
KW  - interleukin 1beta
KW  - vasopressin
KW  - animal cell
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - cell nucleus
KW  - cell size
KW  - controlled study
KW  - densitometry
KW  - drug inhibition
KW  - drug potentiation
KW  - hypophysis cell
KW  - hypothalamus hypophysis adrenal system
KW  - immunocytochemistry
KW  - male
KW  - morphometrics
KW  - nonhuman
KW  - paracrine signaling
KW  - rat
N2  - Interleukin 1? (IL-1?) stimulates the hypothalamic-pituitary- adrenal axis, however the autoparacrine effects of IL-1?, on pituitary ACTH-producing cells and the relations among IL-1? and Arg-vasopressin or corticosterone have not yet been analyzed. To address these effects, pituitary monolayer cultures were treated with IL-1?, Arg-vasopressin and corticosterone, either alone or in combined treatment, and an immunocytochemical, morphometric and densitometric study of ACTH-positive cells was carried out. IL-1? significantly increased the percentage and cellular and nuclear size of ACTH-positive cells, and similar effects were found following treatment with Arg-vasopressin. Combined treatment with IL-1? and Arg-vasopressin increased these parameters more than in separate treatments. Corticosterone decreased these parameters and blocked the effects of IL-1? and Arg-vasopressin in the combined treatments. In sum, our results suggest that IL-1? is an auto-paracrine regulator of pituitary ACTH-producing cells, that it reinforces the stimulatory effects of Arg-vasopressin and that corticosterone is an inhibitory regulator of the auto-paracrine effects of IL-1? and Arg-vasopressin.
ER  -