TY  - JOUR
A1  - ,  
T1  - Immunocytochemical and morphometric evidence of the existence of different cellular populations reactive to r-GHRH in the rat pituitary gland
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2002
VL  - 6
SP  - 1
EP  - 8
UR  - http://www.eurjanat.com/web/paper.php?id=02010001
KW  - growth hormone
KW  - growth hormone releasing factor
KW  - animal tissue
KW  - article
KW  - cell size
KW  - cell structure
KW  - female
KW  - hypophysis
KW  - hypophysis cell
KW  - immunocytochemistry
KW  - immunoreactivity
KW  - male
KW  - morphometrics
KW  - neuroanatomy
KW  - nonhuman
KW  - rat
N2  - In order to characterize the pituitary cells reactive for r-GRF in the adult rat, an immunocytochemicaI and morphometric study was made of the cells immunopositive for r-GHRH and those immunopositive for GH and for GHRH using a double labelling method. The existence of two populations of r-GRF-immunoreactive cells was observed; one of these was only stained with specific serum against r-GRF and the other was immunopositively stained for GH and for r-GHRH. Morphometrically, in both sexes the GH-immunoreactive cells were seen to have a cellular size that was significantly larger (p<0.01) than the r-GHRH-immunoreactive cells. The cells that were only stained for r-GHRH were significantly smaller than those immunoreactive for GH and r-GHRH. Our findings suggest the existence of at least two cellular populations in the pituitary gland of the adult rat able to react with anti-r-GHRH serum.
ER  -