TY - JOUR
A1 - ,
T1 - Testosterone alters membrane binding of progesterone in male rat brains
JO - Eur. J. Anat.
SN - 1136-4890
Y1 - 2001
VL - 5
SP - 37
EP - 45
UR - http://www.eurjanat.com/web/paper.php?id=01010037
KW - bovine serum albumin
KW - estradiol benzoate
KW - progesterone
KW - sodium iodide i 125
KW - testosterone
KW - amygdaloid nucleus
KW - animal experiment
KW - animal tissue
KW - article
KW - autoradiography
KW - central nervous system
KW - gonadectomy
KW - hormone action
KW - hypothalamus
KW - image analysis
KW - male
KW - medial preoptic area
KW - membrane binding
KW - nonhuman
KW - radioiodination
KW - rat
KW - sexual behavior
KW - thalamus midline nucleus
N2 - The steroid progesterone (P) is important in control of male sexual behaviors. In this study binding sites for P conjugated to bovine serum albumin (P-BSA) were analyzed in the brains of gonadectomized male rats that were implanted with silastic capsules either filled with testosterone or empty. Frozen brains were sliced, thaw mounted on microscope slides, and incubated with radiolabeled P-BSA (P-[125I-BSA]) alone or with 1000 fold P-BSA competitor. Microscope slides were then dried, dipped in photographic emulsion and placed in light-tight boxes for four days before being developed and counterstained. Grain densities over cells were then analyzed with an image analysis system. Testosterone treatment significantly increased specific binding of P-[125I-BSA] in the caudal medial basal hypothalamus, the paraventricular nucleus-anterior hypothalamus, and the medial preoptic area. Testosterone treatment significantly decreased P-[125I-BSA] binding in the amygdala. There was no significant effect of testosterone in the rostral medial basal hypothalamus. Testosterone treatment changes P-[125I-BSA] binding in several areas that are important for male sexual behavior suggesting a function for P-[125I-BSA] binding sites in endocrine systems and behavior.
ER -