TY  - JOUR
A1  - ,  
T1  - Enzymatic digestion of neural tube fluid proteoglycans leads to brain growth disruption
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2000
VL  - 4
SP  - 161
EP  - 167
UR  - http://www.eurjanat.com/web/paper.php?id=00030161
KW  - hyaluronidase
KW  - proteoglycan
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - brain growth
KW  - cell division
KW  - cell survival
KW  - embryo
KW  - extracellular matrix
KW  - immunocytochemistry
KW  - mitosis
KW  - neural tube
KW  - neuroblast
KW  - neuroepithelium
KW  - nonhuman
N2  - The expansive force generated by the positive pressure of the neural tube fluid confined inside brain vesicles has been shown to be a key factor during the earliest stages of brain morphogenesis and development of chick and rat embryos. In previous studies, we demonstrated the existence in these species of an intracavity extracellular matrix rich in proteoglycans that could be involved in the regulation of the expansive process. Our results show that the enzymatic digestion of the intracavity proteoglycans by testicular hyaluronidase selectively disrupts the expansive process of brain vesicles, significantly reduces the rate of mitosis in the brain neuroepithelium, and increases the number of the apoptotic cells, leading to a decrease in neuroepithelial volume. These results support the hypothesis that intracerebral proteoglycans play a relevant role in the regulation of the expansive process of the brain primordium in rat embryos that they could be involved in regulating the survival and replication of neuroblasts.
ER  -