TY  - JOUR
A1  - ,  
T1  - Neuropeptide Y and 1,25-dihydroxyvitamin D3 receptors colocalize in neurons of the rat cerebral cortex
JO  - Eur. J. Anat.
SN  - 1136-4890
Y1  - 2000
VL  - 4
SP  - 7
EP  - 13
UR  - http://www.eurjanat.com/web/paper.php?id=00010007
KW  - antibody
KW  - calcitriol receptor
KW  - neuropeptide Y receptor
KW  - neurosteroid
KW  - animal cell
KW  - animal experiment
KW  - animal tissue
KW  - article
KW  - brain cortex
KW  - cingulate gyrus
KW  - entorhinal cortex
KW  - female
KW  - hypothalamus
KW  - immunocytochemistry
KW  - immunofluorescence
KW  - immunoreactivity
KW  - interneuron
KW  - male
KW  - motor cortex
KW  - nerve cell
KW  - nonhuman
KW  - prefrontal cortex
KW  - protein expression
KW  - pyramidal nerve cell
KW  - pyriform cortex
KW  - rat
KW  - sensory cortex
N2  - Using immunocytochemistry we studied the distribution of 1,25-dihydroxyvitamin D3 receptors (VDR) in series of vibratome sections of intact rat brains. With subsequent immunofluorescence we investigated whether calcitriol target neurons express neuropeptide Y (NPY). Single pyramidal cells in the primary motor cortex showed nuclear staining for VDR. A fraction of these cells also contained cytoplasmic NPY immunofluorescence. About 20% of the interneurons in layers 2 and 4 throughout the cerebral cortex stained for VDR, and 10% of these cells were NPY positive. Similar findings were observed in the prefrontal and cingulate cortex, the sensory cortex, the entorhinal cortex and the piriform cortex. In the CA2 and CA3 regions of the hippocampus, numerous non-pyramidal cells and basket cells were seen to be VDR immunoreactive. A fraction of these neurons stained with the NPY antibody. The hypothalamus showed numerous NPY positive perikarya and widely distributed NPY networks. However, no coexistence of VDR and NPY could be observed in this region. Our findings further emphasize that calcitriol may be another important neurosteroid with distinct target areas throughout the brain.
ER  -